这些细胞通常较小,形状不规则,可以在培养基中轻松混合和分散!示例:Farage、Jurkat等!贴壁细胞(AdherentCells):特性:贴壁细胞附着于培养皿表面,通常形成单层或多层细胞群.它们需要培养皿的表面提供附着点,通常会形成可观察的细胞单层.示例:A54HepG2等!半贴壁细胞(Semi-AdherentCells):特性:半贴壁细胞既可以在液体培养基中悬浮,又可以附着于培养皿表面!它们通常在培养基中的一部分细胞悬浮,一部分细胞附着!
常见应用有肿瘤球和血管生成.多种细胞构成,体外培养条件下具有某种程度的组织或器官的功能和结构特征。可模拟真实器官的生物功能!如iPSCs来源类器官。低血清培养基货号产品名称(中文)产品名称(英文)1001内皮细胞培养基ECM(EndothelialCellMedium)1001-NG内皮细胞培养基-不含谷氨酰胺ECM-NG(EndothelialCellMedium-NG)1001-GF内皮细胞培养基-不含葡萄糖ECM-GF(EndothelialCellMedium-GF)1021内皮细胞培养基-大鼠ECM-r(EndothelialCellMedium-Rat)1101平滑肌细胞培养基SMCM(SmoothMuscleCellmedium)1201周边细胞培养基PM(PericyteMedium)1231周边细胞培养基-小鼠PM-m(PericyteMedium-m)1401脑膜细胞培养基MenCM(MeningealCellMedium)1531神经前体细胞的分化培养基NPCDM(NeuralPrecursorCellDifferentiationMedium)1621少突胶质细胞培养基OM(OligodendrocyteMedium)1631少突胶质前体细胞分化培养基OPCDM(OligodendrocytePrecursorCellDifferentiationMedium)1701雪旺细胞培养基SCM(SchwannCellMedium)1801星形胶质细胞培养基AM(AstrocyteMedium)1811星形胶质细胞条件培养基ACM(AstrocyteconditionedMedium)---100ml1831动物星形胶质细胞培养基AM-a(AstrocyteMedium-animal)1901小胶质细胞培养基MM(MicrogliaMedium)1921巨噬细胞培养基MaM(MacrophageMedium)2201黑色素细胞培养基MelM(MelanocyteMedium)2211黑色素细胞培养基-2MelM-2(MelanocyteMedium-TPAfree)2301成纤维细胞培养基FM(FibroblastMedium)2331成纤维细胞培养基-2FM-2(FibroblastMedium-2)3201肺泡上皮细胞培养基AEpiCM(AlveolarEpithelialCellMedium)3501骨骼肌细胞培养基SkMCM(SkeletalMuscleCellMedium)3911胸腺上皮细胞培养基TyEpiCM(ThymicEpithelialCellMedium)4101上皮细胞培养基EpiCM(EpithelialCellMedium)4131动物上皮细胞培养基EpiCM-a(EpithelialCellMedium-animal)4201肾系膜细胞培养基MCM(MesangialCellMedium)4521睾丸支持细细胞培养基SerCM(SertoliCellMedium)4601成骨细胞培养基ObM(OsteoblastMedium)4611成骨细胞矿化培养基ObMM(OsteoblastMineralizationMedium)4651软骨细胞培养基CM(ChondrocyteMedium)4701滑膜细胞培养基SM(SynoviocyteMedium)5201肝细胞培养基HM(HepatocyteMedium)5301星形细胞培养基SteCM(StellateCellMedium)6201心肌细胞培养基CMM(CardiacMyocyteMedium)6591小梁网细胞培养基TMCM(TrabecularMeshworkCellMedium)7121滋养层细胞培养基TM(TrophoblastMedium)7201脂肪细胞培养基AdM(AdipocyteMedium)7211前脂肪细胞培养基PAM(PreadipocyteMedium)7221前脂肪细胞分化培养基PADM(PreadipocyteDifferentiationMedium)7501间充质干细胞培养基MSCM(MesenchymalStemCellMedium)M1811-57小鼠星形胶质细胞条件培养基MACM-57(MouseAstrocyteConditionedMediumfromC57BL/6),100mlR1811大鼠星形胶质细胞条件培养基RACM(RatAstrocyteConditionedMedium),100ml!
ScienCell培养基价格
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细胞培养概述什么是细胞培养?细胞培养是一种实验室技术,用于生长和繁殖细胞!这个过程通常在培养皿或生物反应器中进行,提供细胞所需的适当环境条件,如温度、营养物质和气体.细胞培养可用于研究细胞的生物学特性、生长、分化、药物测试以及生物工程应用,是科学研究和工业生产的重要工具!细胞有哪些种类,各有什么特性?按生长习惯可分为三类:悬浮细胞、贴壁细胞和半贴壁细胞。悬浮细胞(SuspensionCells):特性:悬浮细胞是在液体培养基中自由悬浮的细胞,它们不附着于培养皿表面.
原装magsphere聚台物颗粒_正宗magsphere试剂_上海牧荣生物科技有限公司
成分:F12包括氨基酸、葡萄糖、核酸、维生素和盐类。另有是DMEM和F12的1:1混合物的DMEM/F-12培养基.如何选择合适的培养基?选择适当的细胞培养基是细胞培养中非常重要的步骤,它会影响细胞的生长速率、状态和实验结果!参考文献和厂家建议:查阅ATCC(AmericanTypeCultureCollection)或相关文献,了解以前研究同一或相似细胞系的人所使用的培养基和条件。细胞及细胞培养物厂商提供的说明都是有用的参考资料.
细胞培养进阶--原代细胞系培养是细胞培养中常规的环节,而细胞从来源又可分为细胞系和原代细胞!从培养系统又可分为常规培养及3D/基质培养系统!不同组织来源细胞所需培养体系差别较大!细胞培养试剂0103Trypsin/EDTA胰酶消化液Trypsin/EDTA,0!25%0143CFM-sfCellFreezingMedium-Serum-Free无血清细胞冻存液0863HEPHeparin肝素7531间充质干细胞-成骨细胞分化培养基MODM(MesenchymalStemCellOsteogenicDifferentiationMedium)7541间充质干细胞-脂肪细胞分化培养基MADM(MesenchymalStemCellAdipogenicDifferentiationMedium)无血清培养基货号产品名称(中文)产品名称(英文)1111平滑肌细胞培养基-无血清SMCM-sf(SmoothMuscleCellMedium-serumfree)1511神经前体细胞分化培养基NPCDM(NeuralPrecursorCellDifferentitionMedium)1521神经细胞培养基NM(NeuronalMedium)1601少突胶质前体细胞培养基OPCM(OligodendrocytePrecursorCellMedium)1611球状少突细胞培养基OsM(OligosphereMedium)1811-sf星形胶质细胞条件培养基-无血清ACM-sf(AstrocyteConditionedMedium-SerumFree),100ml2101角质细胞培养基KM(KerotinocyteMedium)2111角质细胞培养基(确定成分)KM-d(KeratinocyteMedium-defined)2311成纤维细胞培养基-无血清FM-sf(FibroblastMedium-serumfree)2561扁桃体上皮细胞培养基TEpiCM(TonsilEpithelialCellMedium)2611口腔角质细胞培养基OKM(OralKeratinocyteMedium)2951结肠上皮细胞培养基CoEpiCM(ColonicEpithelialCellMedium)3211支气管上皮细胞培养基BEpiCM(BronchialEpithelialCellMedium)3231小气道上皮细胞培养基SAEpiCM(SmallAirwayEpithelialCellMedium)4121上皮细胞培养基-2EpiCM-2(EpithelialCellMedium-2)4321尿道上皮细胞培养基UCM(UrothelialCellsMedium)4411前列腺上皮细胞培养基PEpiCM(ProstateEpithelialCellsMedium)4511睾丸间质细胞培养基LCM(LeydigCellMedium)5501造血细胞培养基HemGM(HematoGroMedium)5801无血清,无滋养层人多能干细胞培养基STEMium(STEMiumHumanPluripotentStemCellGrowthMedium)5881小鼠胚胎成纤维细胞-条件培养基MEF-cm(MouseEmbryonicFibroblasts-ConditionedMedium)-100ml6101心肌细胞培养基-无血清CMM-sf(CardiacMyocyteMedium-serumfree)6511角膜上皮细胞培养基CEpiCM(CornealEpithelialCellMedium)7061子宫颈上皮细胞培养基CerEpiCM(CervicalEpithelialCellMedium)7311卵巢上皮细胞培养基OEpiCM(OvarianEpithelialCellMedium)7511间充质干细胞培养基MSCM-sf(MesenchymalStemCellMedium-serumfree)7551间充质干细胞-软骨细胞分化培养基MCDM(MesenchymalStemCellChondrogenicDifferentiationMedium)细胞生长添加物(生长因子)1052ECGSEndothelialCellGrowthSupplement内皮细胞生长添加物无动物成分培养基2121角质细胞培养基KM-acf(KeratinocyteMedium-animalcomponentfree)2321成纤维细胞培养基FM-acf(FibroblastMedium-animalcomponentfree)5521造血细胞培养基HemGM-ACF(HematoGroMedium–AnimalComponentFree)5821人多能干细胞培养基STEMium-acfTM(STEMiumHumanPluripotentStemCellGrowthMedium-animalcomponentfree)7521间充质干细胞培养基MSCM-acf(MesenchymalStemCellMedium-animalcomponentfree)生长因子和细胞因子102-01rhSCF重组人干细胞因子细胞培养进阶-3D培养/类器官目前可提供的3D培养技术支持在3D培养中,细胞可以在更接近其自然环境的条件下生长,形成复杂的三维结构!
RPMI-1640(RoswellParkMemorialInstituteMedium1640)RPMI-1640是由Moore等人1966年开发的,多用于淋巴细胞和肿瘤细胞的培养.成分:RPMI-1640是一种富含氨基酸、维生素和微量元素的培养基。F12(HamsF-12NutrientMixture)F12培养基是由Ham于1965年开发的!多用于MDCK、神经胶质细胞、成纤维细胞、人内皮细胞。