fishersci.com),or2mLdeepwellmicrotiterplate(Product#AB-1127http://www。fishersci!com)For384wellformat—384well(40μLwellcapacity)cyclingplate(forexample:Hard-ShellBioRadPCRplate#HSP-3801orABgeneproduct#AB-1111http://www。

　　beckmancoulter。com)PlateSeals,AdhesiveorHeat•Forexample:Product#AB-3739;http://www.fishersci。comLiquidHandlingRoboticsoraMultichannelHandPipette•RecommendedTheAgencourtAMPureXPPCR*purificationsystemutilizesBeckmanCoulter’ssolid-phasereversibleimmobilization(SPRI)paramagneticbeadtechnologyforhigh-throughputpurificationofPCRamplicons!

　　AgencourtAMPureXPutilizesanoptimizedbuffertoselectivelybindDNAfragments100bpandlargertoparamagneticbeads。Excessprimers,nucleotides,salts,andenzymescanberemovedusingasmplewashingprocedure。TheresultisamorepurifiedPCRproduct。

　　AMPureXPisusedinseveralNextGenerationSequencingApplicationsandissupportedbytheorganizationswhichdevelopedtheseprotocols.ThisprotocolisintendedforthefollowingProducts•A63880AMPureXP5mL•A63881AMPureXP60mL•A63882AMPureXP450mLTheAMPureXPpurificationprocedureishighlyamenabletoBeckmanCoulterBiomekautomationplatformsbecauseitutilizesmagneticseparationandrequiresnocentrifugationorvacuumfiltration。

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　　AgencourtAMPureXPpurifiedproductscanbeusedinthefollowingapplications:•PCR•Sequencing•Genotyping•FragmentAnalysis•PrimerWalking•CloningNOTEAMPureXPissupportedforSNP0byAffymetrix!Forinquiriesregardingthisapplication,pleasecall1-888-362-2447oryourlocalAffymetrixsupportorganization。

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