WorkingUnderRNaseFreeConditions:RNasesareubiquitousandgeneralprecautionsshouldbefollowedinordertoaoidtheintroductionofcontaminatingnucleasesduringtheAgencourtRNACleanXPprocedure.ThemostcommonsourcesofRNasecontaminationarehands,dustparticles,andcontaminatedlaboratoryinstruments,solutionsandglassware。
ForbestresultsshakethereagentwelluntilallofthebeadsarecompletelyinsuspensionandaliquotAgencourtRNACleanXPintoRNasefreecontainers.Donotpourremainingreagentbackintothestoragecontainer。MixAgencourtRNACleanXPwellbeforeuse!
AgencourtRNACleanXPutilizesanoptimizedbuffertoselectivelybindRNAorcDNAtoparamagneticbeads!Excessoligonucleotides,nucleotides,salts,andenzymescanberemovedusingasimplwashingprocedure!Theresultingpurifiedproductcanbeusedinthefollowingapplications:PCR1andRT-PCRProbesformicroarrayormacroarrayRNaseprotectionassaysTransfectionforRNAiexperimentscDNAsynthesisandlabelingMaterialsSuppliedintheAgencourtRNACleanXPKit:AgencourtRNACleanXPMagneticParticleSolutionStoreat4oCuponarrival,forupto12months!
WhenworkingwithRNA,thefollowingproceduresshouldbefollowedtolimitRNasecontamination:AlwaysworkwithglovedhandsandchangeglovesfrequentlyUseRNasefree,filteredpipettetipsforpipettingwheneverpssibleUsededicatedRNasefreeequipment,e.
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abgene!com/]Liquidhandlingroboticsoramulti-channelhandpipetteReagents:Fresh70%ethanol,RNasefree(Note:70%ethanolishygroscopic.Fresh70%ethanolshouldbepreparedforoptimalresults)Reagentgradewater,RNasefree[AmericanBioanalyticalProduct#AB02128;http://www!